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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia; Embrapa Unidades Centrais. |
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Data corrente: |
21/09/2007 |
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Data da última atualização: |
05/04/2016 |
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Tipo da produção científica: |
Documentos |
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Autoria: |
FERREIRA, A. P.; CAMPELLO, E. F. C.; FRANCO, A. A.; RESENDE, A. S. de. |
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Afiliação: |
ADAILTON PEREIRA FERREIRA, UFRRJ; EDUARDO FRANCIA CARNEIRO CAMPELLO, CNPAB; AVILIO ANTÔNIO FRANCO, PESQUISADOR APONSENTADO DA EMBRAPA AGROBIOLOGIA; ALEXANDER SILVA DE RESENDE, CNPAB. |
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Título: |
Uso de leguminosas arbóreas fixadoras de nitrogênio na recuperação de áreas degradadas pela mineração de areia no pólo produtor de Seropédica/Itaguaí. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
Seropédica: Embrapa Agrobiologia, 2007. |
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Páginas: |
31 p. |
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Série: |
(Embrapa Agrobiologia. Documentos, 236). |
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ISSN: |
1517-8498 |
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Idioma: |
Português |
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Conteúdo: |
Aqüífero Piranema; Histórico da mineração em Seropédica e Itaguaí; Processo produtivo da extração de areia; Recuperação das áreas degradadas utilizando medidas biológicas; Plantio; Avaliações de altura, diâmetro e volume de serapilheira 24 meses após o plantio; Avaliações biométricas; Quantidade de serapilheira existente; Estimativas de custos da recuperação. |
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Palavras-Chave: |
Código florestal; Degradação do solo; Forestry codice; Soil deterioration; Soil recuperation; Solo degradado. |
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Thesagro: |
Deterioração do Solo; Recuperação do Solo. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CNPAB-2010/34395/1/doc236.pdf
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Marc: |
LEADER 01271nam a2200277 a 4500
001 1628434
005 2016-04-05
008 2007 bl uuuu u0uu1 u #d
022 $a1517-8498
100 1 $aFERREIRA, A. P.
245 $aUso de leguminosas arbóreas fixadoras de nitrogênio na recuperação de áreas degradadas pela mineração de areia no pólo produtor de Seropédica/Itaguaí.
260 $aSeropédica: Embrapa Agrobiologia$c2007
300 $a31 p.
490 $a(Embrapa Agrobiologia. Documentos, 236).
520 $aAqüífero Piranema; Histórico da mineração em Seropédica e Itaguaí; Processo produtivo da extração de areia; Recuperação das áreas degradadas utilizando medidas biológicas; Plantio; Avaliações de altura, diâmetro e volume de serapilheira 24 meses após o plantio; Avaliações biométricas; Quantidade de serapilheira existente; Estimativas de custos da recuperação.
650 $aDeterioração do Solo
650 $aRecuperação do Solo
653 $aCódigo florestal
653 $aDegradação do solo
653 $aForestry codice
653 $aSoil deterioration
653 $aSoil recuperation
653 $aSolo degradado
700 1 $aCAMPELLO, E. F. C.
700 1 $aFRANCO, A. A.
700 1 $aRESENDE, A. S. de
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
27/12/2017 |
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Data da última atualização: |
03/01/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
POLESE, V.; SOARES, C. de P.; SILVA, P. R. A. da; ARAUJO, J. L. S. de; BALDANI, J. I.; VIDAL, M. S. |
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Afiliação: |
VALÉRIA POLESE, UFRRJ; LEITON DE PAULA SOARES, UFRRJ; PAULA RENATA ALVES DA SILVA, UFRRJ; JEAN LUIZ SIMOES DE ARAUJO, CNPAB; JOSE IVO BALDANI, CNPAB; MARCIA SOARES VIDAL, CNPAB. |
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Título: |
Selection and validation of reference genes for RT-qPCR indicates that juice of sugarcane varieties modulate the expression of C metabolism genes in the endophytic diazotrophic Herbaspirillum rubrisubalbicans strain HCC103. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Antonie van Leeuwenhoekv, 110, n. 12, p. 1555-1568, 2017. |
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ISSN: |
1572-9699 |
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DOI: |
https://doi.org/10.1007/s10482-017-0906-7 |
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Idioma: |
Inglês |
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Conteúdo: |
Quantitative reverse transcription PCR (RT-qPCR) is an important tool for evaluating gene expression. However, this technique requires that specific internal normalizing genes be identified for different experimental conditions. To date, no internal normalizing genes are available for validation of data analyses for Herbaspirillum rubrisubalbicans strain HCC103, an endophyte that is part of the sugarcane consortium inoculant. This work seeks to identify and evaluate suitable reference genes for gene expression studies in HCC103 grown until middle log phase in sugarcane juice obtained from four sugarcane varieties or media with three different carbon sources. The mRNA levels of five candidate genes (rpoA, gyrA, dnaG, recA and gmK) and seven target genes involved in carbon metabolism (acnA, fbp, galE, suhB, wcaA, ORF_0127.0101 and _0127.0123) were quantified by RT-qPCR. Analysis of expression stability of these genes was carried out using geNorm and Normfinder software. The results indicated that the HCC103 dnaG and gyrA genes are the most stable and showed adequate relative expression level changes among the different sugarcane juices. The highest expression level was seen for ORF_0127.0101, which encodes a sugar transporter, in juice from sugarcane variety RB867515 and glucose as the carbon source. The suhB gene, encoding SuhB inositol monophosphatase, had a higher relative expression level on 0.5% glucose, 100% sugarcane juice from variety RB867515 and 0.5% aconitate. Together the results suggest that dnaG and gyrA genes are suitable as reference genes for RT-qPCR analysis of strain HCC103 and that juice from different sugarcane varieties modulates the expression of key genes involved in carbon metabolism. MenosQuantitative reverse transcription PCR (RT-qPCR) is an important tool for evaluating gene expression. However, this technique requires that specific internal normalizing genes be identified for different experimental conditions. To date, no internal normalizing genes are available for validation of data analyses for Herbaspirillum rubrisubalbicans strain HCC103, an endophyte that is part of the sugarcane consortium inoculant. This work seeks to identify and evaluate suitable reference genes for gene expression studies in HCC103 grown until middle log phase in sugarcane juice obtained from four sugarcane varieties or media with three different carbon sources. The mRNA levels of five candidate genes (rpoA, gyrA, dnaG, recA and gmK) and seven target genes involved in carbon metabolism (acnA, fbp, galE, suhB, wcaA, ORF_0127.0101 and _0127.0123) were quantified by RT-qPCR. Analysis of expression stability of these genes was carried out using geNorm and Normfinder software. The results indicated that the HCC103 dnaG and gyrA genes are the most stable and showed adequate relative expression level changes among the different sugarcane juices. The highest expression level was seen for ORF_0127.0101, which encodes a sugar transporter, in juice from sugarcane variety RB867515 and glucose as the carbon source. The suhB gene, encoding SuhB inositol monophosphatase, had a higher relative expression level on 0.5% glucose, 100% sugarcane juice from variety RB867515 and 0.5% aconitate. Toget... Mostrar Tudo |
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Palavras-Chave: |
Internal normalizing; Organic acids; Relative expression; Sugar transport. |
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Thesaurus NAL: |
nitrogen fixation. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02682naa a2200265 a 4500
001 2083708
005 2018-01-03
008 2017 bl uuuu u00u1 u #d
022 $a1572-9699
024 7 $ahttps://doi.org/10.1007/s10482-017-0906-7$2DOI
100 1 $aPOLESE, V.
245 $aSelection and validation of reference genes for RT-qPCR indicates that juice of sugarcane varieties modulate the expression of C metabolism genes in the endophytic diazotrophic Herbaspirillum rubrisubalbicans strain HCC103.$h[electronic resource]
260 $c2017
520 $aQuantitative reverse transcription PCR (RT-qPCR) is an important tool for evaluating gene expression. However, this technique requires that specific internal normalizing genes be identified for different experimental conditions. To date, no internal normalizing genes are available for validation of data analyses for Herbaspirillum rubrisubalbicans strain HCC103, an endophyte that is part of the sugarcane consortium inoculant. This work seeks to identify and evaluate suitable reference genes for gene expression studies in HCC103 grown until middle log phase in sugarcane juice obtained from four sugarcane varieties or media with three different carbon sources. The mRNA levels of five candidate genes (rpoA, gyrA, dnaG, recA and gmK) and seven target genes involved in carbon metabolism (acnA, fbp, galE, suhB, wcaA, ORF_0127.0101 and _0127.0123) were quantified by RT-qPCR. Analysis of expression stability of these genes was carried out using geNorm and Normfinder software. The results indicated that the HCC103 dnaG and gyrA genes are the most stable and showed adequate relative expression level changes among the different sugarcane juices. The highest expression level was seen for ORF_0127.0101, which encodes a sugar transporter, in juice from sugarcane variety RB867515 and glucose as the carbon source. The suhB gene, encoding SuhB inositol monophosphatase, had a higher relative expression level on 0.5% glucose, 100% sugarcane juice from variety RB867515 and 0.5% aconitate. Together the results suggest that dnaG and gyrA genes are suitable as reference genes for RT-qPCR analysis of strain HCC103 and that juice from different sugarcane varieties modulates the expression of key genes involved in carbon metabolism.
650 $anitrogen fixation
653 $aInternal normalizing
653 $aOrganic acids
653 $aRelative expression
653 $aSugar transport
700 1 $aSOARES, C. de P.
700 1 $aSILVA, P. R. A. da
700 1 $aARAUJO, J. L. S. de
700 1 $aBALDANI, J. I.
700 1 $aVIDAL, M. S.
773 $tAntonie van Leeuwenhoekv, 110$gn. 12, p. 1555-1568, 2017.
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